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SPECIES AFFINITIES

Species Affinity for Protein A** Affinity for Protein L*

Human

++++

++++

Horse

++

+/-

Cow

++

-

Pig

+++

++++

Sheep

+/-

-

Goat

+/-

-

Rabbit

++++

+

Chicken

-

++

Hamster

+

++++

Guinea Pig

++++

++

Rat

+/-

++++

Dog

++

+

Mouse

++

++++

*de Chateau et al. (1993) Scand. J. Immunol. 37, 399-405. It should be noted that rProtein L binding is restricted to specific subclasses of the κ domain. Thus the affinity indicated in the Tables is not general for the IgG subclass but accounts only for those antibodies carrying the right kappa light chain.

**Protein A binds to the fraction of Ig that carries the VHIII isotype (approximately 10%).



 

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ANTIBODY AFFINITIES

Monoclonal Antibody Affinity for Protein A** Affinity for Protein L*

Human IgG1

++++

++++

Human IgG2

++++

++++

Human IgG3

-

++++

Human IgG4

++++

++++

Rat IgG1

-

++++

Rat IgG2a

-

++++

Rat IgG2b

-

++++

Rat IgG2c

+

++++

Rat IgM

+/-

+

Mouse IgG1

+

++++

Mouse IgG2a

++++

++++

Mouse IgG2b

+++

++++

Mouse IgG3

++

++++

Mouse IgM

+/-

++

Human ScFv

++/0

++++

Human Fab

++/0

++++

Human Fc

++

-

Human κ

-

++++

Human λ

-

-

Human IgM

++/-

++++

Human IgA

++/-

++++

Human IgE

++/-

++++

Human IgD

++/-

++++

*de Chateau et al. (1993) Scand. J. Immunol. 37, 399-405. It should be noted that rProtein L binding is restricted to specific subclasses of the κ domain. Thus the affinity indicated in the Tables is not general for the IgG subclass but accounts only for those antibodies carrying the right kappa light chain.

**Protein A binds to the fraction of Ig that carries the VHIII isotype (approximately 10%).



 

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